WebAug 10, 2024 · The CyTOF workflow implemented for this study has been designed with several specific considerations to reduce sample usage, streamline sample processing, and minimize experimental variability . Whole-blood samples are first stained at the site of collection using a commercial lyophilized 30-marker panel designed to identify all major ... WebApr 14, 2024 · CyTOF data were processed using a CyTOF workflow, and analyses were implemented using the CATALYST package in R . CyTOF data were arcsinh (inverse hyperbolic sine) transformed with a cofactor of 5, and batch-corrected using the quantile normalization method for the pooled distribution of each batch ...
CyTOF workflow: differential discovery in high-throughput …
Webthis workflow facilitates robust, highly standardized CyTOF immune monitoring in studies involving multiple clinical sites that cannot accommodate complex sample processing … WebMay 26, 2024 · Our workflow offers different analysis paths, including association of cell type abundance with a phenotype or changes in signalling markers within specific … how did lainey wilson get her start
Single-cell mass cytometry of microglia in major depressive …
WebOur workflow offers different analysis paths, including association of cell type abundance with a phenotype or changes in signaling markers within specific subpopulations, or differential analyses of aggregated signals. ... CyTOF datasets are usually much larger and thus to keep running times reasonable, one may use a subset of cells; for ... WebDec 17, 2024 · Nonetheless, CyTOF offers a high dimension of parameters measured per cell, with current pan- els using ~40 parameters and the promise of up to 100. Throughput of CyTOF is lower , at the rate of ... WebJun 29, 2024 · Mass cytometry (CyTOF) represents one of the most powerful tools in immune phenotyping, allowing high throughput quantification of over 40 single parameters at single-cell resolution. However, wide deployment of CyTOF-based immune phenotyping studies are limited by complex experimental workflows and the need for specialized … how many shops does lush have