Flp-in cho
WebMar 22, 2024 · Cystic fibrosis (CF) is a genetic disease that affects the lungs and digestive system by making fluids and mucus thick and sticky. This causes persistent lung infections that progressively limit the ability to breathe. Children with CF also suffer from digestive problems that prevent their bodies from breaking down and absorbing food, leading ... WebThe Flp-In™ System allows for stable integration and expression of your gene of interest to deliver single-copy isogenic cell lines. Flp-In™ expression involves introduction of a Flp …
Flp-in cho
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WebThe Flp-In-CHO Cell Line was created by transfecting CHO cells with pFRT/ lac Zeo2 and selecting for Zeocin™-resistant clones Flp-In-CHO cell lines work well with Flp-In vectors that express gene from CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO™, and pSecTag/FRT/V5-His-TOPO™) WebAug 24, 2024 · Stable Flp-In™ CHO expression cell lines were generated by co-transfection of Flp-In™ expression construct (pcDNA™5/FRT/TO_FSHR) and the pOG44 plasmid (Invitrogen #V6005-20). Co-transfection was done using lipid-mediated transfection (Lipofectamine; Invitrogen #11668, Thermo Fisher Scientific Inc., Waltham, MA, USA …
WebFeb 13, 2024 · Flp‐In CHO‐METKO cells (METKO), wild‐type MET (MET WT)–, or 11N all 11Q mutant MET (11N all 11Q)–transfected Flp‐In CHO‐METKO cells were stained with FITC‐conjugated anti‐MET antibody and analyzed by flow cytometry. C, Confocal microscopy analysis of MET (red) and nuclear (cyan). Flp‐In CHO‐METKO cells … WebInvitrogen™ Flp-In™-CHO Cell Line. Designed for rapid generation of stable cell lines that express protein of interest from Flp-In expression vector. Includes: 3 x 10 6 cells are …
WebAll of the Flp-In ™ cell lines (except Flp-In -CHO; see the following section) contain a single integrated FRT site and stably express the lacZ-Zeocin™ fusion gene from the pFRT/lacZeo plasmid under the control of the SV40 early promoter (see the following diagram). The location of the FRT site in each WebFlp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster …
WebThe Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/ lac Zeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains …
WebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain a single stably … fluorine building blockWebOct 25, 2024 · Flp-In-CHO:huOX40 cells as well as Flp-In-CHO:vector cells generated in-house (Pieris) were cultivated in Ham’s F12 medium (Gibco, #31765-027) with 10% FBS and 500 µg/ml hygromycin B. All mammalian cell lines were grown at 37°C and 5% CO 2. Goat anti-rabbit IgG-PE conjugate was purchased from Dianova/Jackson … fluorine coating cookwareWebFlp-In™Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™host cell lines that stably express the lacZ-Zeocin™fusion gene from pFRT/lacZeo or pFRT/lacZeo2. Each cell line contains a single integrated FRT site as confirmed by Southern blot analysis. greenfield psychiatryWebFlp-In™ T-REx™-293 3 × 106 cells, frozen R780-07 Flp-In™ Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™ host cell lines that stably express the lacZ-Zeocin™ fusion gene from pFRT/lacZeo or pFRT/lacZeo2 (Flp-In™-CHO). Each cell line contains a single integrated FRT site fluorine chaingreenfield protocol contrast allergyWebSeveral Flp-In™ host cell lines which stably express the lacZ-Zeocin™ fusion gene and contain a single integrated FRT site are available (see page 7 for ordering information). To express your gene of interest in 293, CV-1, CHO, 3T3, BHK, or Jurkat cells, you may want to use one of the Flp-In™ host cell lines to establish fluorine containing tonerWebJun 21, 2024 · Among different recombinases, the Flp/FRT system has been extensively applied in CHO cells to express monoclonal antibodies 35 and recombinant proteins 34, 36 probably due to its high... greenfield psychology yeppoon