WebObjectives are designed to image specimens either with air or a medium of higher refractive index between the front lens and the specimen. The field of view is often quite limited, and the front lens element of the objective is placed close to the specimen with which it must … WebJun 20, 2016 · However, high NA objective, large exposure field of view (26mm×1.5∼2mm), and strict lithography performance are severely restricted or conflict with each other, which makes the optical design ...
What is Microscope Field of View? – Microscope Clarity
WebApr 12, 2024 · Imaging large, cleared samples requires microscope objectives that combine a large field of view (FOV) with a long working distance (WD) and a high numerical aperture (NA). Ideally, such objectives should be compatible with a wide range of immersion media, which is challenging to achieve with conventional lens-based objective designs. Here we … WebWhen using higher magnification objectives, field of view is being restricted for no reason. By using lower magnification objectives, changing from a 100x, 1.30 NA objective to a 40x, 1.30 NA objective, field of view is increased by 450%. That represents a 450% increase in sample area with no loss in resolution. iphone storage is full message
ZEISS Microscopy Online Campus Microscopy Basics The Point …
http://labrigger.com/blog/2010/06/29/high-na-low-mag/ WebThe NA is calculated from the refractive index in front of the objective and the half-angle (θ) of light that the lens transmits from the sample towards the detector.. NA = n * sinθ The more open the aperture, the more angles of light that can pass through the lens to the sample, and the higher the NA.At its largest, the angle would be defined by the radius and … WebThe ideal point spread function ( PSF) is the three-dimensional diffraction pattern of light emitted from an infinitely small point source in the specimen and transmitted to the image plane through a high numerical aperture ( NA) objective. It is considered to be the fundamental unit of an image in theoretical models of image formation. iphone storage keeps crashing