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Pirf3 antibody

WebbFigure Legend Snippet: 293ET cells were transfected with FLAG-tagged wt IKKε or mutants as indicated on top and were fixed at 20 h post-transfection. Fixed cells were stained with anti-FLAG and anti-IRF3 antibodies and were observed by confocal microscopy. Green, red and blue fluorescence in merged figures (top panels) indicate the endogenous IRF3, … WebbAnti Phosphorylated Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS ... Arrows indicate the cells were positive stained with anti-EV71 3D and anti-pIRF3 antibodies. ( B ) Percentages of pIRF3-positive and negative cells were counted in EV71-infected cells.

Phospho-IRF-3 (Ser396) Antibody Cell Signaling …

Webb11 apr. 2024 · Cells were lysed after 1.5 h and IRF3 was immunoprecipitated with anti-IRF3 antibody (1:100 dilution, 655702, 12A4A35, BioLegend). The level of TBK1 co-immunoprecipitated was assessed with ... Webb23 mars 2024 · Cells were stained with anti-IRF3 antibody (red) and Hoechst nuclear stain (blue), and images were overlaid (right). (B) Quantification of anti-IRF3 antibody and Hoechst stain [inset in (A)] along the line scan (dashed … raport zus ria co to jest https://decobarrel.com

MAP Kinase-Mediated Licensing of Interferon Regulatory Factor …

Webb10 mars 2011 · To determine whether IRF3, similarly to IRF8, binds within the IFN-β promoter, EMSA using the antibody against IRF3 was performed. The heterocomplex band, which was supershifted by antibodies specific for IRF8 ( Figure 2 E), was similarly inhibited by the antibody against IRF3 (supplemental Figure 5A), indicating that the DNA-protein … WebbInteraction between IRF3 2A and p-S366-STING was visualized under confocal microscopy, using PLA with an anti-HA monoclonal antibody for IRF3 and an anti–p-S366 rabbit polyclonal antibody (top). The cell in the top right was further subjected to a z -stack collection, which obtains and combines multiple images at different focal distances … Webb10 sep. 2024 · lated IRF3 (Ser396), total STING, Actin (Cell Signaling) and IRF3 (Abcam) as shown in Table 1 . Anti-rabbit HRP label secondary antibody (Abcam) and Clarity Max ™ western ECL drone dji com rtk

Selective autophagy controls the stability of transcription factor IRF3 …

Category:Targeting IRF3 as a YAP agonist therapy against gastric cancer

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Pirf3 antibody

Anti-phospho-IRF-3 (Ser396) Antibody 07-582-I - EMD Millipore

Webb22 okt. 2024 · The input and IP complexes were analyzed by western blotting using anti-IRF3, anti-Myc or anti-GAPDH antibodies. (C) PK15 cells were transfected with empty vector (0.5 μg) or Myc-US3 (0.5 μg) for 24 h, then cells were collected for western blotting using anti-IRF3, anti-Myc or anti-GAPDH antibodies. WebbWhole cell extracts from CTRL or IRF3 −/− T98G cells treated or not with CPT for 72 h were analyzed by WB using indicated antibodies. THP-1 CTRL , THP-1 cGAS −/− and THP-1 STING −/− were challenged or not with dsDNA for …

Pirf3 antibody

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Webb12 mars 2015 · Antibody against mice IFNR1 was purchased from Leinco Technologies (St. Louis, MO). The antibodies specific to Myc, IRF3, TBK1, phospho-IRF3 and PCNA were from Cell Signaling Technology (Beverly, MA). Antibody specific to Cullin 1 was from OriGene Technologies (Rockville, MD). The antibody for Flag and VSV G protein were … WebbDetection of Mouse IRF3 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse IRF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4454) followed by HRP-conjugated Anti-Goat IgG ...

WebbPhospho-IRF3 (Ser386) Antibody (PA5-78025) in WB. Western Blot using Phospho-IRF3 (Ser386) Polyclonal Antibody ( Product # PA5-78025 ). Untreated (–) and treated (+) … WebbEach IRF3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our IRF3 Antibodies can be used in a variety of model species: Human, Mouse. …

WebbPurified anti-IRF3 Antibody. Total lysates (15 µg protein) from Daudi (lane 1) and A20 (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF3 antibody clone 12A4A35 (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary ... WebbPhospho-IRF-3 (Ser396) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser396. This antibody does not cross-react with other family …

Webb10 dec. 2024 · Unfortunately, the antibody used to detect IRF3 phosphorylation at S396 by flow cytometry (Cell Signaling D6O1M) did not work well in immunoblots in our hands. Further, the antibody detecting IRF3 phosphorylation at S386 by immunoblot (Abcam ab76493) doesn’t work well in flow cytometry, in our hands.

WebbRabbit IRF3 Rabbit pAb (A11118), validaed in WB,IHC,IF/ICC,ChIP,IP and tested in Human,, Mouse,, Rat. 100% Guaranteed. ABclonal provides free trial antibodies for target detection. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. rapor ukdwWebbThis antibody is predicted to react with Monkey. Target Information IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent … raport zus u2WebbPhospho-IRF3 (Ser396) Antibody (MA5-14947) in WB Frontiers in microbiology 2024 - FIGURE 2 Effects of Set-Met on the expression of MAVS protein and the phosphorylation … raporu