WebbFigure Legend Snippet: 293ET cells were transfected with FLAG-tagged wt IKKε or mutants as indicated on top and were fixed at 20 h post-transfection. Fixed cells were stained with anti-FLAG and anti-IRF3 antibodies and were observed by confocal microscopy. Green, red and blue fluorescence in merged figures (top panels) indicate the endogenous IRF3, … WebbAnti Phosphorylated Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS ... Arrows indicate the cells were positive stained with anti-EV71 3D and anti-pIRF3 antibodies. ( B ) Percentages of pIRF3-positive and negative cells were counted in EV71-infected cells.
Phospho-IRF-3 (Ser396) Antibody Cell Signaling …
Webb11 apr. 2024 · Cells were lysed after 1.5 h and IRF3 was immunoprecipitated with anti-IRF3 antibody (1:100 dilution, 655702, 12A4A35, BioLegend). The level of TBK1 co-immunoprecipitated was assessed with ... Webb23 mars 2024 · Cells were stained with anti-IRF3 antibody (red) and Hoechst nuclear stain (blue), and images were overlaid (right). (B) Quantification of anti-IRF3 antibody and Hoechst stain [inset in (A)] along the line scan (dashed … raport zus ria co to jest
MAP Kinase-Mediated Licensing of Interferon Regulatory Factor …
Webb10 mars 2011 · To determine whether IRF3, similarly to IRF8, binds within the IFN-β promoter, EMSA using the antibody against IRF3 was performed. The heterocomplex band, which was supershifted by antibodies specific for IRF8 ( Figure 2 E), was similarly inhibited by the antibody against IRF3 (supplemental Figure 5A), indicating that the DNA-protein … WebbInteraction between IRF3 2A and p-S366-STING was visualized under confocal microscopy, using PLA with an anti-HA monoclonal antibody for IRF3 and an anti–p-S366 rabbit polyclonal antibody (top). The cell in the top right was further subjected to a z -stack collection, which obtains and combines multiple images at different focal distances … Webb10 sep. 2024 · lated IRF3 (Ser396), total STING, Actin (Cell Signaling) and IRF3 (Abcam) as shown in Table 1 . Anti-rabbit HRP label secondary antibody (Abcam) and Clarity Max ™ western ECL drone dji com rtk